Explain the importance of including a control group in analytical epidemiological studies, provide an example.Why is it important to measure the NAD+ levels in body fluids?

According to the authors, what is the problem with currently used measurement method of NAD+(eNAD+ or iNAD+)and what is the advantage of their developed assay over it?

Explain the reactions which is employed in the developed assay and how the researchers used it to measure the eNAD+ levels

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